Gliomas are the most typical central nervous system malignancies and current with vital morbidity and mortality. Treatment modalities are presently restricted to surgical resection, chemotherapy and radiotherapy. Increases in survival charge over the earlier a long time are negligible, additional pinpointing an unmet scientific want in this area. There is a continuous battle with the event of efficient glioma diagnostics and therapeutics, largely resulting from a mess of things, together with the presence of the blood-brain barrier and vital intertumoural and intratumoural heterogeneity.
Importantly, there’s a lack of dependable biomarkers for glioma, notably in aiding tumour subtyping and measuring response to therapy. There is a necessity for biomarkers that might each overcome the complexity of the illness and permit for a minimally invasive technique of detection and evaluation. This is a complete assessment evaluating the potential of present mobile, proteomic and molecular biomarker candidates for glioma. Significant hurdles confronted in glioma diagnostics and therapy are additionally mentioned right here. The Fused (Fu) kinase is a key transducer of Hedgehog signaling, however its related substrates have remained obscured because of the problem of acquiring lively Fu for in vitro kinase assay.
Based on the mechanism of Fu activation in vivo, we engineered a constitutively lively Fu and expressed it in Sf9 cells utilizing the baculovirus system. The kinase was affinity purified and utilized for in vitro kinase assay utilizing recombinant GST-fusion proteins as substrates to establish Fu-specific phosphorylation websites. For full particulars on the use and execution of this protocol, please check with Han et al. (2019).
How and When Does Outcrossing Occur in the Predominantly Selfing Species Medicago truncatula?
Empirical research on pure populations of Medicago truncatula revealed selfing charges increased than 80%, however by no means as much as 100%. Similarly, a number of research of predominantly selfing species present variability in the extent of residual outcrossing between populations and additionally between temporal samples of the identical inhabitants. However, these research measure international selfing charges on the scale of the inhabitants and we have no idea whether or not there may be intra-population variation and how outcrossing occasions are distributed, between genotypes, vegetation, flowers, or seeds.
Theoretical research predict the upkeep of residual outcrossing in extremely selfing species resulting from environmental (e.g., pollen biology) and/or genetic determinants and decompositions of the variation in outcrossing charge utilizing experimental information may be very informative to check these hypotheses. Here, we concentrate on one pure inhabitants of M. truncatula in order to explain exactly its mating system. In specific, we investigated the determinants of the selfing charge by testing for seasonal differences (environmental determinism) and variations between genotypes (genetic determinism).
We measured selfing charges in maternal progenies from vegetation collected broadly throughout a pure inhabitants. For every plant, we collected pods from flowers produced initially and on the finish of the flowering season to check for a seasonal variation in the outcrossing charge. For every collected offspring, we additionally estimated the chance that it was issued from a self-fertilization occasion and assessed the genetic part of variation of this mating system measure.
We discovered a big, albeit small, improve in outcrossing charge in progenies collected on the finish [t m = 0.137 (SD = 0.025)] in comparison with these collected initially [t m = 0.083 (0.016)] of the flowering season. A major between genotypes variation in selfing charge was additionally detected, ensuing in a heritability of 9% for the speed of residual outcrossing. Altogether, our work exhibits that regardless of a predominantly selfing reproductive mode, M. truncatula shows variation in residual outcrossing charge, and that this trait is probably going below a fancy determinism combining environmental and genetic elements. We talk about the evolutionary implications of our outcomes for the inhabitants.
The intersect of genetics, surroundings, and microbiota in asthma-perspectives and challenges
In bronchial asthma, a good portion of the interplay between genetics and surroundings happens by way of microbiota. The proposed mechanisms behind this interplay are complicated and at occasions contradictory. This assessment covers latest developments in our understanding of this interplay: the “microbial speculation” and the “farm impact”; the position of endotoxin and genetic variation in sample recognition programs; the interplay with allergen publicity; the extra involvement of host intestine and airway microbiota; the position of viral respiratory infections in interplay with the 17q21 and CDHR3 genetic loci; and the significance of in utero and early-life timing of exposures.
We suggest a unified framework for understanding how all these phenomena work together to drive bronchial asthma pathogenesis. Finally, we level out some future challenges for continued analysis in this area, in specific the necessity for multiomic integration, in addition to the potential utility of bronchial asthma endotyping. We characterised fish eDNA compositions utilizing speedy, low-volume filtering with replicate and management samples scaled for a single Illumina MiSeq movement cell, utilizing the mitochondrial 12S ribosomal RNA locus for taxonomic profiling.
 Sodium citrate dihydrate |
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| GK0137-500G | Glentham Life Sciences | 500 g | EUR 52 |
Sodium citrate dihydrate |
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| GK0137-5KG | Glentham Life Sciences | 5 kg | EUR 142 |
) Sodium chloride (Molecular Biology Grade) |
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| CE205 | GeneOn | 500 g | EUR 52 |
Sodium chloride (Molecular Biology Grade) |
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| CE206 | GeneOn | 1 kg | EUR 59 |
Sodium chloride (Molecular Biology Grade) |
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| CE207 | GeneOn | 5 kg | EUR 103 |
 Sodium citrate, trisodium salt, dihydrate |
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| CB0035 | Bio Basic | 500g | EUR 61.48 |
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) NADP - sodium salt (Molecular Biology Grade) |
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| CE200 | GeneOn | 250 mg | EUR 77 |
NADP - sodium salt (Molecular Biology Grade) |
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| CE201 | GeneOn | 1 g | EUR 159 |
) XTT sodium salt (Molecular Biology Grade) |
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| CE250 | GeneOn | 100 mg | EUR 174 |
XTT sodium salt (Molecular Biology Grade) |
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| CE251 | GeneOn | 500 mg | EUR 510 |
 Ammonium citrate tribasic |
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| GL7832-100G | Glentham Life Sciences | 100 g | EUR 56 |
Ammonium citrate tribasic |
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| GL7832-1KG | Glentham Life Sciences | 1 kg | EUR 178 |
Ammonium citrate tribasic |
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| GL7832-250G | Glentham Life Sciences | 250 g | EUR 82 |
Ammonium citrate tribasic |
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| GL7832-25G | Glentham Life Sciences | 25 g | EUR 44 |
Ammonium citrate tribasic |
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| GL7832-500G | Glentham Life Sciences | 500 g | EUR 118 |
 Ammonium citrate, tribasic |
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| AB0060 | Bio Basic | 250g | EUR 60.44 |
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 Urea, suitable for molecular biology |
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| GE1210-1KG | Glentham Life Sciences | 1 kg | EUR 89 |
Urea, suitable for molecular biology |
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| GE1210-500G | Glentham Life Sciences | 500 g | EUR 64 |
 Magnesium citrate tribasic hydrate |
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| GK3304-1KG | Glentham Life Sciences | 1 kg | EUR 86 |
Magnesium citrate tribasic hydrate |
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| GK3304-500G | Glentham Life Sciences | 500 g | EUR 62 |
 Sucrose, GlenBiol, suitable for molecular biology |
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| GC3201-1KG | Glentham Life Sciences | 1 kg | EUR 75 |
 Zinc citrate dihydrate |
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| GE1507-1KG | Glentham Life Sciences | 1 kg | EUR 138 |
) BCIP (Molecular Biology Grade) |
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| CE108 | GeneOn | 250 mg | EUR 63 |
BCIP (Molecular Biology Grade) |
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| CE109 | GeneOn | 1 g | EUR 90 |
) CHAPS (Molecular Biology Grade) |
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| CE114 | GeneOn | 1 g | EUR 55 |
CHAPS (Molecular Biology Grade) |
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| CE115 | GeneOn | 5 g | EUR 131 |
CHAPS (Molecular Biology Grade) |
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| CE116 | GeneOn | 25 g | EUR 410 |
) DAPI (Molecular Biology Grade) |
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| CE117 | GeneOn | 5 mg | EUR 60 |
DAPI (Molecular Biology Grade) |
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| CE118 | GeneOn | 25 mg | EUR 133 |
DAPI (Molecular Biology Grade) |
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| CE119 | GeneOn | 100 mg | EUR 319 |
) Dimethylsulfoxide (Molecular Biology Grade) |
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| CE120 | GeneOn | 100 ml | EUR 55 |
Dimethylsulfoxide (Molecular Biology Grade) |
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| CE121 | GeneOn | 500 ml | EUR 92 |
) DTT (Molecular Biology Grade) |
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| CE131 | GeneOn | 5 g | EUR 78 |
DTT (Molecular Biology Grade) |
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| CE132 | GeneOn | 10 g | EUR 111 |
DTT (Molecular Biology Grade) |
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| CE133 | GeneOn | 25 g | EUR 203 |
) Glycine (Molecular Biology Grade) |
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| CE158 | GeneOn | 1 kg | EUR 70 |
Glycine (Molecular Biology Grade) |
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| CE159 | GeneOn | 5 kg | EUR 190 |
) HEPES (Molecular Biology Grade) |
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| CE171 | GeneOn | 100 g | EUR 82 |
HEPES (Molecular Biology Grade) |
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| CE172 | GeneOn | 500 g | EUR 224 |
HEPES (Molecular Biology Grade) |
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| CE173 | GeneOn | 1 kg | EUR 354 |
) Lysozyme (Molecular Biology Grade) |
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| CE188 | GeneOn | 1 g | EUR 59 |
Lysozyme (Molecular Biology Grade) |
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| CE189 | GeneOn | 10 g | EUR 206 |
) NAD (Molecular Biology Grade) |
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| CE196 | GeneOn | 1 g | EUR 60 |
NAD (Molecular Biology Grade) |
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| CE197 | GeneOn | 5 g | EUR 138 |
) NBT (Molecular Biology Grade) |
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| CE209 | GeneOn | 1 g | EUR 103 |
NBT (Molecular Biology Grade) |
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| CE210 | GeneOn | 5 g | EUR 300 |
) Tris (Molecular Biology Grade) |
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| CE237 | GeneOn | 500 g | EUR 89 |
Tris (Molecular Biology Grade) |
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| CE238 | GeneOn | 1 kg | EUR 128 |
Tris (Molecular Biology Grade) |
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| CE239 | GeneOn | 5 kg | EUR 446 |
) Tween20 (Molecular Biology Grade) |
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| CE242 | GeneOn | 1 l | EUR 89 |
) Water (Molecular Biology Grade) |
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| CE243 | GeneOn | 500 ml | EUR 52 |
Water (Molecular Biology Grade) |
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| CE244 | GeneOn | 1 l | EUR 56 |
 Sodium phosphate, tribasic, dodecahydrate |
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| SC8240 | Bio Basic | 500g | EUR 62.92 |
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 Sodium citrate |
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| B7298-500000 | ApexBio | 500 g | EUR 112 |
 Water, Ultrapure Molecular Biology Grade |
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| 41024-4L | Biotium | 4L | EUR 121 |
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Description: Minimum order quantity: 1 unit of 4L |
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) Ammonium sulfate (Molecular Biology Grade) |
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| CE105 | GeneOn | 250 g | EUR 46 |
Ammonium sulfate (Molecular Biology Grade) |
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| CE106 | GeneOn | 1 kg | EUR 60 |
Ammonium sulfate (Molecular Biology Grade) |
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| CE107 | GeneOn | 5 kg | EUR 128 |
) Bis-Acrylamid (Molecular Biology Grade) |
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| CE110 | GeneOn | 50 g | EUR 79 |
Bis-Acrylamid (Molecular Biology Grade) |
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| CE111 | GeneOn | 250 g | EUR 216 |
) Formamide deionized (Molecular Biology Grade) |
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| CE145 | GeneOn | 500 ml | EUR 73 |
Formamide deionized (Molecular Biology Grade) |
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| CE146 | GeneOn | 1 l | EUR 100 |
) Glycerol 87 % (Molecular Biology Grade) |
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| CE154 | GeneOn | 1 l | EUR 78 |
) Glycerol waterfree (Molecular Biology Grade) |
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| CE155 | GeneOn | 500 ml | EUR 65 |
Glycerol waterfree (Molecular Biology Grade) |
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| CE156 | GeneOn | 1 l | EUR 85 |
Glycerol waterfree (Molecular Biology Grade) |
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| CE157 | GeneOn | 2.5 l | EUR 142 |
) Guanidine - Hydrochloride (Molecular Biology Grade) |
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| CE160 | GeneOn | 100 g | EUR 78 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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| CE161 | GeneOn | 250 g | EUR 128 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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| CE162 | GeneOn | 500 g | EUR 194 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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| CE163 | GeneOn | 1 kg | EUR 294 |
) Guanidine Thiocyanate (Molecular Biology Grade) |
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| CE164 | GeneOn | 100 g | EUR 72 |
Guanidine Thiocyanate (Molecular Biology Grade) |
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| CE165 | GeneOn | 500 g | EUR 160 |
Guanidine Thiocyanate (Molecular Biology Grade) |
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| CE166 | GeneOn | 1 kg | EUR 256 |
) Urea Crystalline (Molecular Biology Grade) |
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| CE167 | GeneOn | 1 kg | EUR 60 |
Urea Crystalline (Molecular Biology Grade) |
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| CE168 | GeneOn | 5 kg | EUR 151 |
) MOPS buffer (Molecular Biology Grade) |
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| CE194 | GeneOn | 100 g | EUR 85 |
MOPS buffer (Molecular Biology Grade) |
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| CE195 | GeneOn | 250 g | EUR 141 |
-Sucrose (Molecular Biology Grade)) D(+)-Sucrose (Molecular Biology Grade) |
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| CE224 | GeneOn | 500 g | EUR 56 |
D(+)-Sucrose (Molecular Biology Grade) |
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| CE225 | GeneOn | 1 kg | EUR 70 |
D(+)-Sucrose (Molecular Biology Grade) |
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| CE226 | GeneOn | 5 kg | EUR 173 |
) Tris - Hydrochloride (Molecular Biology Grade) |
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| CE234 | GeneOn | 250 g | EUR 83 |
Tris - Hydrochloride (Molecular Biology Grade) |
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| CE235 | GeneOn | 500 g | EUR 120 |
Tris - Hydrochloride (Molecular Biology Grade) |
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| CE236 | GeneOn | 1 kg | EUR 186 |
) TritonX-100 (Molecular Biology Grade) |
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| CE240 | GeneOn | 500 ml | EUR 56 |
TritonX-100 (Molecular Biology Grade) |
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| CE241 | GeneOn | 1 l | EUR 66 |
 Tween 20, Molecular Biology Grade |
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| T9100-010 | GenDepot | 100ml | EUR 72 |
Tween 20, Molecular Biology Grade |
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| T9100-050 | GenDepot | 500ml | EUR 111 |
Tween 20, Molecular Biology Grade |
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| T9100-100 | GenDepot | 1L | EUR 134 |
 Water, distilled, GlenBiol™, suitable for molecular biology |
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| GK8512-1L | Glentham Life Sciences | 1 l | EUR 77 |
 Agarose, low EEO, GlenBiol, suitable for molecular biology |
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| GE6258-100G | Glentham Life Sciences | 100 g | EUR 181 |
 Double distilled for Molecular Biology) Phenol, (Carbolic acid) Double distilled for Molecular Biology |
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| PD0252 | Bio Basic | 500g | EUR 160.49 |
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) Tolmetin (sodium dihydrate) |
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| HY-B1489 | MedChemExpress | 100mg | EUR 119 |
 Sodium nitroprusside dihydrate |
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| GT2671-100G | Glentham Life Sciences | 100 g | EUR 64 |
Sodium nitroprusside dihydrate |
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| GT2671-250G | Glentham Life Sciences | 250 g | EUR 98 |
Sodium nitroprusside dihydrate |
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| GT2671-25G | Glentham Life Sciences | 25 g | EUR 44 |
Sodium nitroprusside dihydrate |
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| GT2671-500G | Glentham Life Sciences | 500 g | EUR 156 |
 Sodium tetrathionate dihydrate |
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| GK8710-100G | Glentham Life Sciences | 100 g | EUR 389 |
 Sodium tartrate dihydrate |
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| GK1687-1KG | Glentham Life Sciences | 1 kg | EUR 108 |
Sodium tartrate dihydrate |
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| GK1687-250G | Glentham Life Sciences | 250 g | EUR 56 |
Sodium tartrate dihydrate |
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| GK1687-500G | Glentham Life Sciences | 500 g | EUR 74 |
 Sodium tungstate dihydrate |
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| GK6152-100G | Glentham Life Sciences | 100 g | EUR 66 |
Sodium tungstate dihydrate |
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| GK6152-250G | Glentham Life Sciences | 250 g | EUR 102 |
Sodium tungstate dihydrate |
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| GK6152-25G | Glentham Life Sciences | 25 g | EUR 47 |
Sodium tungstate dihydrate |
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| GK6152-500G | Glentham Life Sciences | 500 g | EUR 158 |
 Sodium tungstate, dihydrate |
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| S0898 | Bio Basic | 250g | EUR 109.16 |
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Sodium nitroprusside dihydrate |
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| SB0867 | Bio Basic | 50g | EUR 70.01 |
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 Sodium phosphate tribasic dodecahydrate, 98% |
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| GK9846-1KG | Glentham Life Sciences | 1 kg | EUR 59 |
Sodium phosphate tribasic dodecahydrate, 98% |
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| GK9846-500G | Glentham Life Sciences | 500 g | EUR 50 |
Sodium phosphate tribasic dodecahydrate, 98% |
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| GK9846-5KG | Glentham Life Sciences | 5 kg | EUR 150 |
 Sodium citrate, anhydrous |
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| GK2264-100G | Glentham Life Sciences | 100 g | EUR 44 |
Sodium citrate, anhydrous |
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| GK2264-1KG | Glentham Life Sciences | 1 kg | EUR 94 |
Sodium citrate, anhydrous |
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| GK2264-250G | Glentham Life Sciences | 250 g | EUR 54 |
Sodium citrate, anhydrous |
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| GK2264-500G | Glentham Life Sciences | 500 g | EUR 67 |
Sodium citrate, anhydrous |
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| GK2264-5KG | Glentham Life Sciences | 5 kg | EUR 261 |
) EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
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| CE135 | GeneOn | 250 g | EUR 60 |
EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
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| CE136 | GeneOn | 500 g | EUR 72 |
EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
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| CE137 | GeneOn | 1 kg | EUR 104 |
EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
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| CE138 | GeneOn | 5 kg | EUR 349 |
-Glucose waterfree (Molecular Biology Grade)) D(+)-Glucose waterfree (Molecular Biology Grade) |
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| CE148 | GeneOn | 500 g | EUR 56 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
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| CE149 | GeneOn | 1 kg | EUR 63 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
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| CE150 | GeneOn | 5 kg | EUR 150 |
) Yeast extract powder (Molecular Biology Grade) |
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| CE169 | GeneOn | 500 g | EUR 111 |
) Hyaluronidase Grade I (Molecular Biology Grade) |
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| CE174 | GeneOn | 1 g | EUR 194 |
Hyaluronidase Grade I (Molecular Biology Grade) |
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| CE175 | GeneOn | 5 g | EUR 767 |
) Magnesium acetate - Tetrahydrate (Molecular Biology Grade) |
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| CE190 | GeneOn | 500 g | EUR 82 |
) NADH - Disodium salt (Molecular Biology Grade) |
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| CE198 | GeneOn | 1 g | EUR 76 |
NADH - Disodium salt (Molecular Biology Grade) |
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| CE199 | GeneOn | 5 g | EUR 204 |
) NADPH - Tetrasodium salt (Molecular Biology Grade) |
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| CE202 | GeneOn | 25 mg | EUR 59 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
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| CE204 | GeneOn | 500 mg | EUR 312 |
 (Molecular Biology Grade)) SSC Buffer (20X) (Molecular Biology Grade) |
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| CE229 | GeneOn | 1 l | EUR 72 |
 Sodium 4-aminosalicylate dihydrate |
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| HY-I0447A | MedChemExpress | 5g | EUR 160 |
 Sodium molybdate dihydrate, 98% |
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| GX5429-1KG | Glentham Life Sciences | 1 kg | EUR 166 |
Sodium molybdate dihydrate, 98% |
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| GX5429-500G | Glentham Life Sciences | 500 g | EUR 107 |
 Sodium dihydrogen phosphate dihydrate |
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| GK8728-100G | Glentham Life Sciences | 100 g | EUR 42 |
Our targets had been to find out: (1) spatiotemporal variation in eDNA abundance, (2) the filtrate wanted to attain robust sequencing libraries, (3) the taxonomic decision of 12S ribosomal sequences in the research surroundings, (4) the portion of the anticipated fish group detectable by 12S sequencing, (5) biases in species restoration, (6) correlations between eDNA compositions and catch per unit effort (CPUE) and (7) the extent that eDNA profiles replicate main watershed options.